The laboratory for immunology and molecular biology of positive strand RNA viruses is engaged in research on pestiviruses and caliciviruses. We investigate the biochemical basis of viral replication and the molecular mechanisms that the viruses use for interaction with the hosts immune system. The results of this work provide a basis for the establishment of virus mutants with altered replication characteristics or impaired ability to interfere with the immune system of the host. The mutants are characterized in detail to better understand the underlying mechanisms and to generate attenuated virus variants that could be used as vaccines. Analysis of the genetic stability of the introduced mutations are conducted to learn more about the potential for reversion and, in consequence, the safety of genetically modified viruses.
Classical swine fever virus, bovine viral diarrhea virus
- mechanisms of viral replication and gene expression
- identification of virulence markers
- analysis of immune modulating mechanisms in the context of pestivirus infections in the natural host
- molecular basis of persistent pestivirus infections