Assessment of Neospora caninum seroprevalence in cattle holdings
To estimate the Neospora caninum seroprevalence in dairy and beef cattle, a cross sectional study was conducted in the German state of Rhineland-Palatinate in collaboration with the Landesuntersuchungsamt Rheinland-Pfalz and the Landeskontrollverband Rheinland-Pfalz e.V.. Within an EU funded project, this study started in 2003 and ended 2004. Out of the farms organized in a dairy farmers organisation (Landeskontrollverband Rheinland-Pfalz e.V.) (n = 2082) 100 dairy herds were randomly selected for participating in the study. Individual milk samples from 4343 cows were taken. The milk samples were examined by an ELISA previously modified and evaluated for the examination of milk samples. The total prevalence in the milk-ELISA was 4.0% (172/4343). The intra-herd prevalence varied from 0% to 42.86%. 35% (35/100) of dairy herds had an intra-herd prevalence of >5% whereas in 15% (15/100) of the herds it was >10%.
In the context of serological examinations on bovine herpes virus type 1 and bovine leucosis virus serum sets of serum samples collected from 106 beef herds were haphazardly selected from those submitted to the investigation centre of state (Landesuntersuchungsamt Rheinland-Pfalz). The total prevalence in the serum-ELISA was 4.9% (131/2680). The intra-herd prevalence varied from 0% to 54.5%. 37% (39/106) of dairy herds had an intra-herd prevalence of >5% whereas in 21% (22/106) of the herds it was ≥10%.
The proportion of dairy or beef herds with intra-herd-prevalences of >5% was at 54.6% or 50.0% in the province Rheinhessen/Pfalz, respectively, at 39.0% or 41.8% in the province Koblenz, respectively, and at 27.1% or 24.3% in the province Trier, respectively (Blumröder et al., 2006).