Today the scientific community produces more data than ever. New methods and technical progress make it possible to store and share theses data. New tools are necessary for a useful exploitation of these data but also assurance of data quality is a prerequisite for any reliable analysis result. The Laboratory for Biocuration works on methods for data curation and puts them into praxis.
GISAID EpiFlu™: Data curation of an Influenza sequence database
GISAID EpiFlu™ (www.gisaid.org) is the world’s most comprehensive publicly accessible database for Influenza sequence data. The technical hosting of the database is realized by the Federal Office for Agriculture and Food (BLE). The Friedrich-Loeffler-Institut carries out scientific plausibility check.
The curator develops and applies new concepts and methods for quality-based data processing together with software experts.
Ad-hoc-de-novo-detection of viral pathogens with adaptive diagnostics for the prevention of epidemics (DetectiVir) : Optimization of in-lab and in-silico methods for metagenomic virus detection
Deployment of a generic diagnostic workflow based on next-generation sequence information and new integrated bioinformatic database approaches for the direct detection of new viral pathogens. The joint project combines molecular nucleic acid-based virus discovery by metagenomic sequencing with ad hoc development of customized serological diagnostics. An integral part will be a new and innovative database application.
The project DetektiVir is a colloborative project within the framework “Civil Security” (www.sifo.de) funded by the BMBF.
The FLI provides the data platform for the Users at https://detektivir.fli.de.
One goal of this collaborative project funded by European Union Horizon 2020 program (www.compare-europe.eu) is to harmonize deep sequencing methods and workflows as a prerequisite for their usage in clinical applications. One of several projects is a pilot study to compare sequencing workflows in a ring trial with samples from three European H5N8 Influenza outbreaks.
In a joint approach with the Reference Laboratory for Avian Influena : Methods for pathogen characterization by deep sequence population analysis.
In a joint approach with the Reference Laboratories for Swine Fever : Deepsequencing of clinical swine feces for pathogen detection and identification of coinfections.