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Institute of Infectology (IMED)

Laboratory for Bee Diseases

The Laboratory is extending the tasks of the National Reference Laboratories to other important parasites and pathogens of honey bees.

Main Research topics and projects

  • Subtyping of Paenibacillus larvae for epidemiological investigations
  • Project partner in the German Bee Monitoring Project (DeBiMo)

Method overview

  • ERIC-Genotyping of Paenibacillus larvae by repPCR and MALDI-TOF MS
  • Identification of Paenibacillus larvae and Melissococcus plutonius by real-time PCR
  • Identification of Vespa velutina by macroscopic examination
  • Phase contrast microscopy for identification of Nosemosis
  • Identification of Nosema apis and Nosema ceranae by real-time PCR
  • Differentiation of Nosema spp. by PCR and restriction enzyme digestion
  • Phase contrast microscopy for identification of infestation with Malpighamoeba mellificae
  • Identification of Acarapis woodi by microscopic examination
  • Molecular identification of RNA-viruses of honey bees by RT-PCR and real-time PCR:
    • Akute Bee Paralysis Virus (ABPV)
    • Black Queen Cell Virus (BQCV)
    • Chronic Bee Paralysis Virus (CBPV)
    • Deformed Wing Virus (DWV)
    • Sacbrood Virus (SBV)