Determination of the pathogen
It has developed a working manual for the diagnosis of bovine genital campylobacter-iosis, the recently updated (July 2010) and was published by the FLI. Currently, the traditional distinction between the two subspecies based on a single phenotypic response (tolerance of 1% glycine).
However, glycine tolerant C. fetus subspecies venerealis variants (referred to as biovar intermedius) have been described. In addition, glycine sensitive C. fetus subspecies fetus isolates observed. Now it appears possible, the differentiation of the two C. fetus subspecies by PCR this is the most methodical progress of recent years undoubtedly.
This test is carried out in our lab. The primers were designated by Hum et al. (1997). Using the primers MG3F and MG4R the PCR resulted in a 764-bp amplicon for both subspecies of C. fetus. The differentiation of both subspecies is done by the primer pair VenSF and VenSR, only for C. fetus subspecies venerealis a 142-bp amplicon is obtained.
The type strains of the two subspecies are used as positive controls, nonspecific reactions were not observed. We continue to hold the traditional phenotypic characterization of C. fetus subspecies is essential to see, but in the PCR a valuable tool to complement and confirm these findings.